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KMID : 0861020170320030029
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Korea Journal of Herbology
2017 Volume.32 No. 3 p.29 ~ p.36
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Antioxidant Activities of Hot Water and Ethanol Extracts from the stem of Sorbus commixta Heal
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Yoo Ji-Hyun
Doh Eun-Soo
Chang Jun-Pok
Kill Ki-Jung
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Abstract
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Objectives : In order to investigate the possibility of Sorbus commixta Heal. stem (SC) as a natural material, antioxidant activities of the hot water and ethanol extracts were examined.
Methods : The samples of SC were pulverized, and fractions were extracted repeatedly three times from hot water and 70% ethanol at room temperature for 2 hours. The antioxidant activities were analyzed from total polyphenol, flavonoid contents, DPPH, ABTS, hydroxyl radical, Fe2+ chelating, and nitrite scavenging activity.
Results : Total polyphenol contents were significantly higher in ethanol extract group (504.39 ㎍/㎎) than in hot water extract group (364.64 ㎍/㎎). Total flavonoid contents were also significantly higher in ethanol extract group (160.09 ㎍/㎎) than in hot water extract group (124.59 ㎍/㎎). DPPH, ABTS, Fe2+chelating were slightly higher in ethanol extract gorups than in hot water extract groups, and increased in a dose-dependent manner. The hydroxyl radical scavenging activity (18.42~23.61%) of ethanol extract groups were shown to be approximately twice higher than that (7.63~10.37%) of hot water extract groups at 12.5~50 ㎍/㎖ concentration. Nitrite scavenging activities of both ethanol and hot-water extract groups were shown to be higher in a dose dependent manner at the concentration of 12.5~50 ㎍/㎖ at pH 3.0 than at pH 1.2, and ethanol extract groups (86.55~96.64%) had higher activity than the hot water extract groups (42.59~92.63%), which was higher than that of the control group antioxidant BHT (72.96~80.11%).
Conclusions : The extracts of SC displayed antioxidant activities which suggested a natural material can be developed to functional material.
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KEYWORD
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Antioxidant, Sorbus commixta, Hot-Water Extract, Ethanol Extracts
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